Novel Validated Chromatographic Method for Determination of Some Anti-hypertensive Drugs

Accurate, precise and reproducible isocratic RP-HPLC method was developed and subsequent validated for the analysis of Torasemide (I), Irbesartan (II) and Olmesartan medoxomil (III) at ambient temperature, using Atlantis 4.6 mm x 250 mm RP-C18 Column, with a flow rate of 1.5 ml.min, and UV. detector at 288 nm and 260 nm for (I) and (II and III), respectively. By adopting the mentioned chromatographic technique, (I) and (III) were determined in the presence of their acidic and alkaline-degradates separately as stability-indicating methods utilizing phosphate buffer pH = 3:acetonitrile (60:40, v/v), phosphate buffer pH = 3.2:acetonitrile (60:40, v/v) as a mobile phase, respectively, while (II) was determined in presence of Hydrochlorothiazide (HCTZ), using phosphate buffer pH = 4:acetonitrile (70 :30, v/v). All the proposed methods were validated according to the International Conference on Harmonization (ICH) guidelines and successfully applied to determine the mentioned studied drugs in pure form, in laboratory prepared mixtures and in pharmaceutical preparations. The obtained results were statistically compared to the reference methods of analysis [for I and "II and III", respectively] and no significant differences were found. [M. Farouk, O. Abd ELAziz, A. Hemdan, M. Shehata. Novel Validated Chromatographic Method for Determination of Some Anti-hypertensive Drugs. Journal of American Science 2010;6(11):476-486]. (ISSN: 1545-1003).